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KMID : 0861020160310060029
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Korea Journal of Herbology
2016 Volume.31 No. 6 p.29 ~ p.35
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Anti-adipogenesis Effects of 3 Herbal Formula on Blood Stasis
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Lee Ho-Young
Shim Eun-Hyoung
Lee Myeong-Soo
Lee Ju-Ah
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Abstract
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Objectives : Blood stasis (BS) is related to be caused by blood circulation and stagnation which are cancer, atherosclerosis and hyperlipidemia in traditional medicine. We extracted 3 kinds of BS formula; Seogakjihwang-tang (SGT), Tonggyuhawlhyul-tang (THT), Hyulbuchukeo-tang (HCT). This study was conducted to investigate whether the 3 kinds of herbal formula extracts have inhibitory efficacy association with anti-adipogenesis.
Methods : To investigate the anti-adipogenesis, we used the mouse fibroblast cell line, 3T3-L1 which differentiated into adipocytes in response to insulin, IBMX and dexamethasone (MDI). Cytotoxicity of herbal formula extracts were examined by CCK-8 kit. Intracellular lipid droplets were detected by Oil-Red-O staining. Triglyceride (TG) and leptin were measure using elisa kit.
Results : The yield of water extracts was 14.62% (SGT), 21.27% (THT), 20.02% (HCT). Lipid accumulation was reduced significantly by 3 kinds of herbal formula compared to control. Especially, THT and HCT decreased lipid droplet, respectively at all concentration. The TG and leptin were also inhibited by 3 kinds of herbal formula. The IC50 of TG were 280.51 ㎍/㎖ (SGT), 52.62 ㎍/㎖ (THT), 313.99 ㎍/㎖ (HCT). The IC50 of leptin were 348.76 ㎍/㎖ (SGT), 164.02 ㎍/㎖ (THT), 257.00 ㎍/㎖ (HCT). THT was better than other herbal formula on anti-adipogenesis.
Conclusion : kinds of herbal formula inhibited adipogenesis in MDI-induced 3T3-L1 adipocytes, as indicated by the significant reduction in TG and leptin concentration without cytotoxicity. Therefore, 3 kinds of herbal formula for BS might act as a therapeutic agent for preventing lipid diseases, such as obesity and atherosclerosis.
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KEYWORD
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Blood stasis, Seogakjihwang-tang (Xijiao Dihuang Decoction), Tonggyuhawlhyul-tang (Tongqiaohuoxue Decoction), Hyulbuchukeo-tang (Xuefuzhuyu decoction), anti-adipogenesis
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